Title: Factors affecting the enzymes reaction rates
Purpose: observing the differences in the reaction because of the different concentration of enzymes
Hypothesis: Higher concentrations of lactase drops will react stronger to the milk. As more lactase is put into the milk, the glucose concentration should be higher.
Materials:
- Five Test Tubes
- Beaker or Graduated Cylinder
- [Lactose] milk
- Lactase
- Glucose test strips
Introduction
In this lab, we will be adding the lactase enzyme to 5 test tubes containing 20ml of milk, and then testing the glucose levels in these test tubes after 10 minutes. We believe the concentration of the enzyme in the milk with affect the glucose levels by increasing them as we add more of the enzyme.
Procedure:
- Put 20mL milk in each test tube
- Put no drops of lactase enzymes in the first test tube, add 2 drops lactase enzymes in the second tube, add 4 drops lactase in test tube 3, 6 drops in tube 4, 8 drops in tube 5
- Wait for 10 minutes
- Use the glucose test strips to see how many glucose is in the test tube
Results (Data + Observations)
| beaker | []enzymes | Initial [] glucose (mmo/L) | Final [] glucose (mmo/L) |
| 1 | 0 drop
|
0 mmo/L | 0 mmo/L |
| 2 | 2 drop
|
0 mmo/L | 14 mmo/L |
| 3 | 4 drops
|
0 mmo/L | 111 mmo/L |
| 4 | 6 drops
|
0 mmo/L
|
28 mmo/L |
| 5 | 8 drops
|
0 mmo/L | 56 mmo/L |
Data Analysis/Conclusion
After conducting our experiment, we noticed that our data is not consistent with what we expected. We thought that there would be an increase in glucose as we added more drops however, the glucose levels fluctuated. It started with an increase from 0-2 drops, then an even larger jump from 2-4 drops. After that, the glucose levels decreased with 6 drops and then rose again with 8 drops. This could have been an error in how we handled the procedure (as mentioned below) and so we cannot make an accurate conclusion based off of this data. Had we received the data that we predicated however, we could have said that our conclusion is that when you add more lactase to lactose, glucose levels rise.
Errors / Improvements
The result of this test is not the same as what we thought, there are several reasons that might cause the data to be inaccurate. First, we should shake the test tubes after we put the enzymes into the milk. By shaking it, it could help to make the reaction happen properly. And we can also reduce the number of drops that we put in each test tube, which could give us a more detailed result