Cheek Cell DNA strand:

Stained Cheek Cells:

 

Disrupted Cheek Cells:

 

Question #1: Which of the above solutions do you believe would be useful in the process of breaking apart all membranes inside of a cells without destroying the strands of DNA?

I believe the first method would be the most effective for disrupting the membrane without destroying the contents of the cell. Although cheek cells are easily removed from the inner linings of the mouth, the DNA strands are fragile and must be handled with extreme care. Nevertheless, I was able to locate and examine the detailed structure of a DNA strand without using a microscope. The saltwater used in the experiment was supposed to make the cheek cells less soluble in water, but in my case did not alleviate the difficulties of relocating the strands in the new test tube. However, it proved to be a viable option for breaking down the cells. Additionally, the third method (disrupting the cells using detergents and soap water) also proved effective. For example, adding soap water to the cheek cells dissolved the membranes and nucleus of the cell, freeing the DNA. However, my group decided to use an acidic solution that completely dissolved the entire cell, rendering it useless.

Question #2: What did you find interesting/new?

Overall, observing the complex structures of the DNA I extracted, and the actual shape of the cells were the most fascinating aspects of these labs. Biology is by far my favorite subject in science, and I enjoyed learning how to use a microscope properly and analyzing the cell disruptions caused by the various solutions provided. Unfortunately, the biology unit last year was cut short, and we did not perform any experiments. I have always enjoyed experiential learning where I am able to immerse myself in a subject by performing hands-on activities. Furthermore, I was also surprised that when the cells were dyed blue that I was able to fully recognize the parts of the cell and locate the cytoplasm, cell membrane, and nucleus. I had fully expected them to appear as confusing blobs I could not decipher and already be destroyed. Lastly, it was also captivating to see the cells break down after adding various solutions since they eventually became translucent and disintegrated when adding an acid.