Posts Tagged ‘replication’

RNA transcription lab – blk x emmi

how is mRNA different from DNA?

DNA is made up of deoxyribose sugar but mRNA is made up of ribose sugar. DNA has thymine as one of the two pyrimidines while mRNA has uracil as its pyrimidines base.

describe the process of transcription

 Initiation:

  • RNA polymerase binds to DNA at a specific sequence of nucleotides

Elongation:

  • Only one of the unmound DNA strands acts as a template for the RNA

Termination:

  • RNA polymerase continues to elongate until it reaches the terminator, a specific sequence of nucleotides that signals the end of transcription

 

in what way was our model inaccurate? how did it do a good job?

the models we made were in accurate because they did not exactly show properly how transcription takes place with the unwinding mid way down he strand, but it did show the helicase and the polmerase which is accurate.

 

 

 

 

 

 

DNA replication lab – blk X

When does DNA replication occur?

replication occurs before cell division.

Name and describe the three steps in DNA replication. Why does the process occur differently on the leading and lagging strands?

1- the helicase unzips and unwinds the dna helix

2- the polmerase does complimentary base pairing for the lagging strand

3- the ligase moves and pairs nucleotides on the adjacent strand

the process is different on the lagging strand because the 5′ 3′ pattern is flipped and the ligase has to work backwards with rna primase

3.  what did you do to show complimentary base pairing? how was this activity good/bad?

to show the base pairing on the adjacent strand and the regular strand, we used the corresponding beads from the leading strand to pair with the lagging strand. this activity was good because it showed the basic steps of replication, but it did not go into depth, which i believe would be more helpful.