Feb
2018
DNA replication lab – blk X
When does DNA replication occur?
replication occurs before cell division.
Name and describe the three steps in DNA replication. Why does the process occur differently on the leading and lagging strands?
1- the helicase unzips and unwinds the dna helix
2- the polmerase does complimentary base pairing for the lagging strand
3- the ligase moves and pairs nucleotides on the adjacent strand
the process is different on the lagging strand because the 5′ 3′ pattern is flipped and the ligase has to work backwards with rna primase
3. what did you do to show complimentary base pairing? how was this activity good/bad?
to show the base pairing on the adjacent strand and the regular strand, we used the corresponding beads from the leading strand to pair with the lagging strand. this activity was good because it showed the basic steps of replication, but it did not go into depth, which i believe would be more helpful.