Protein synthesis

Transcription

mRNA is different from DNA in multiple ways. From its structure to its functions, there are numerous differences.

For structural differences, mRNA is single-stranded and short. Where DNA has the classic double helix (Two sugar-phosphate backbones) and has a hefty 85 million nucleotides, mRNA only has one strand (One sugar-phosphate backbone) of about 1000 nucleotides. Finally, mRNA contains a different nucleotide base; where DNA has the base Thymine, RNA has the base Uracil.

For functional differences, DNA has the “blueprint” to create proteins, but mRNA is the method in which DNA’s message gets to the ribosomes. DNA is too large to exit the nucleus, so the smaller mRNA allows the protein synthesis to happen.

During transcription, there are three main steps, and these steps are mostly similar to the ones of DNA Replication. The first step is unwinding/unzipping. Here, the helix unwinds into the ladder form and then unzips.

The next step of RNA transcription is complimentary base pairing. A sugar-phosphate backbone containing the sugar Ribose and not the sugar Deoxyribose comes in to create the complimentary strand of one DNA backbone strand (shown as blue pipe-cleaner). It will have the same complimentary bases with one exception: instead of the base Thymine, mRNA will have the base Uracil. The mRNA (shown as the red pipe-cleaner) will create a complimentary strand of the leading strand only because this strand contains the essential information on how to build proteins. The enzyme RNA Polymerase will facilitate this process, creating H-bonds between the nucleotides and bonding the backbone and the nucleotides. While this is happening, the lagging strand of DNA in not involved. This is modelled in the image below.

The final step is called separation. This happens when the RNA Polymerase is finished transcribing DNA’s message onto the mRNA, and the mRNA will simply float away. The DNA leading and lagging strands will rejoin and reform the double helix, whereas the mRNA will be checked for any errors in the base sequence. There, it will be modified so that all the bases are correct, and unnecessary bases will be taken away. mRNA can now exit the nucleus towards the ribosomes and DNA is in its original double helix with nothing changed to it.

Our activity was able to clearly demonstrate how the process of Transcription works. The diagrams nicely show how each step looks, and gives us a nice visual representation. There were, however, a couple of ways the model could have been different so that the process is better depicted. One way was that the strand of DNA was equal in length to the strand of mRNA, so it was difficult to show the process of the double helix unzipping at regions where the transcription occurs. Consequentially, we had to depict the process of unzipping as a full “unzip” and not just a partial “unzip”.

Translation

Before translation occurs, transcription must first be completed. RNA Polymerase will complete the transcription of DNA’s message onto mRNA. This will allow the ribosomes to read this message.

The first process is called initiation. This is when the transcribed mRNA approaches the ribosome. The ribosome has two sites: the P-site in the left, and the A-site in the right. The mRNA base sequence is divided into groups of three bases called codons. These codons will translate into a certain amino acid. The mRNA will “look” for the codon AUG – this is the “Start Codon”. This codon will enter the P-site, and the next codon will be in the A-site. A tRNA will enter the P-site.

tRNA is a special type of RNA that has a specific anti-codon. Every tRNA will have one specific anti-codon, and also carries the amino acid it corresponds to. For example, when the ribosome reads the codon AUG, it will call for the tRNA with the anti-codon UAC that carries the amino acid Methionine.

The next process is called elongation. This is when the amino acids brought in by the tRNA begin to create a polypeptide. Once the codon in the P-site is read, the codon in the A-site will also be read. From there, the tRNA carrying the right amino acid will enter the ribosome. Now, both sites are filled, so the amino acid in the P-site will bond with the amino acid in the A-site.

Now, the tRNA in the P-site has no amino acid, which allows it to leave. Due to the P-site being empty, the mRNA will shift to the left; this puts the tRNA with the two amino acids in the P-site, and the A-site empty for a new amino acid to enter. This process continues, creating a long chain of amino acids.

The final step of translation is called termination. There are certain codons that will not correspond to an amino acid due to there not being a tRNA with the proper anti-codon. This is a message to the ribosome to stop adding amino acids to the polypeptide chain. There are three variations of these codons that stop the elongation process, which are fittingly named “Stop Codons”. In this case, the ribosome will let go of the mRNA and will let go of the amino acid polypeptide. The protein that was intended by DNA’s message is now completely created and ready to function.

 

This model of translation is quite accurate to the actual process of translation. It provided a nice visual representation of how the process occurs, which also made it easier to understand. One way that we could improve this model could maybe be by making the amino acids something more block-y and 3-D. The flat paper made it difficult to envision the primary structure that would soon twist and turn into the globular form of a tertiary/quaternary structure protein.

DNA and Protein Synthesis – Luka Mladenovic

DNA is created by having two backbones of sugar-phosphate groups, as we can see with the blue pipe cleaner (the Deoxyribose sugar) and the pink beads (the phosphate groups). The order must always go in an alternating order of sugar-phosphate or vice versa. Each sugar-phosphate group has a nitrogen-containing group sticking out. The group of sugar-phosphate-base is the basic monomer for DNA which is called a nucleotide. Many nucleotides are bonded together to form a strand of DNA.

There are four different bases: Adenine (Yellow bead), Guanine (Purple bead), Cytosine (Green bead), Thymine (Blue bead). Adenine and Guanine are purines, which mean they have two rings. This is represented by Adenine and Guanine having two beads. Cytosine and Thymine are pyrimidines, which mean they have one ring. This is represented by Cytosine and Thymine having one bead. The bases follow a “protocol” called complimentary base pairing, which means that a certain base will only bond with another certain base. Adenine (A) will only bond with Thymine (T), and Guanine (G) will only bond with Cytosine (C).

The first strand will start with three specific bases, and this is the same for all strands of DNA. The sequence goes T, A, C. This means that the opposite strand will begin with the sequence A, T, G. From there, the sequence will differ for different strands of DNA, and there will be complimentary base pairing for the opposite strand.

When the strand is completed, the opposite, complimentary strand will be created following two different “rules”. The first is that the sugar-phosphate groups will be facing the opposite way. This means that if the first, original strand had a sequence of sugar-phosphate, the opposite strand will have a sequence of phosphate-sugar. This is called an antiparallel strand. The second is that the bases will be complimentary to the first strand following the complimentary base pairing rule. This means that the complimentary strand will begin with the three bases A, T, G. The bases in the two strands will H-bond with one another, creating a form that looks similar to a ladder.

Now that the ladder form is complete, the strand must twist into a double helix. DNA molecules have a right-handed twist and is twisted evenly throughout. The form of DNA that most people know is now complete.

The model of DNA and its characteristics are very well demonstrated by this activity. The diagram is almost identical to what the textbook models suggest. In the textbook diagrams, it is hard to see that the backbone is made of a sugar-phosphate group since it just looks like a backbone strand. The activity could be more accurate by putting the bases closer to one another, farther from the sugar-phosphate backbone. The last suggestion for this activity would be twisting the strand into a more accurate double helix. The pipe cleaners and the beads were prone to falling apart if they were twisted too much. If there was a certain way to allow the strand to twist more without breaking, that would be better.

DNA Replication

Cells, much like ourselves, age and become defunct. In that case, the cells must reproduce via cell division. However, the DNA in the parent cell nucleus must also be copied into the new cell nucleus. This happens via DNA replication. DNA must be copied and remade into a new strand of the same DNA before any cell division can occur. Therefore, DNA replication occurs when cell division happens.

There are three steps during DNA replication.

The first step is called the unwinding, or the unzipping process. In this step, the double helix untwists itself back into the ladder form and is unwounded/unzipped. This unzipping is facilitated by an enzyme called DNA Helicase, which breaks the H-bonds between nitrogen-containing bases so that the ladder is separated into the two separate backbones.

The second step is called Complimentary Base Pairing. This is the process where the unzipped strands begin to find their new “partner” strands. Nucleotides of all types are floating around in the nucleus. Complimentary bases to the original strand are brought in and connected into fragments. This process is facilitated by the enzyme DNA Polymerase. The direction in which DNA Polymerase reads and strand and brings in complimentary bases is strictly one-way; from Carbon-3 to Carbon-5 to be exact. For the leading strand, the sequence of the backbone molecules goes 5 to 3, which means that it follows a phosphate-sugar sequence. This means that as the DNA unravels, the DNA Polymerase can continue to read the strand and bring in complimentary bases. For the lagging strand, it is the opposite. The sequence of the backbone molecules goes 3 to 5 (sugar-phosphate sequence). This means that as the DNA unravels, the DNA Polymerase reads the strand in the same direction as the strand, which means that the DNA Polymerase has to return to the beginning of the unravelling Replication Fork and read again.

The final step is called joining. This is the process where fragments of complementary bases are “glued” together into a long backbone strand. This is facilitated by the enzyme DNA Ligase. From there, once the long strand of complementary bases are completed, they form H-bonds with their complementary bases of the original strand. Now, the original strand has successfully formed two new DNA strands with two separate backbones. They will twist again and form a double helix.

It was difficult to show the individual nucleotides floating towards the replication site since we had not thought of cutting up the blue pipe cleaner to demonstrate this. Due to this, we had modeled the process of Complimentary Base Pairing and Joining virtually together. Furthermore, we did not have the best idea as to where the DNA Ligase should have been, so after looking at various diagrams on Google, we decided to simply put the Ligase near the fragment of complementary bases. This would have been an inaccurate way of showing this model. Also, for the unravelling of the DNA, we were not entirely sure if the TAC sequence of the leading strand had to be the first to unravel, or the last to unravel. We ended up guessing that it would be the beginning that unravels first, and we’re still not sure whether that is the case or not.

However, there were many accurate representations in the model that we made. For example, the DNA Helicase looked mostly like the pictures of DNA Helicase that we saw in the notes or in textbooks. The general model looked like the diagrams in the notes as well. The way that we had shown the H-bond between the bases (hooking the white pipe cleaners together) made it relatively simple for us to unhook the “bond” and separate them to show the process of Complementary Base Pairing.

Neuron/synapse Structure and Function – Psychology 12 – Luka Mladenovic

There are 3 types of nerve cells. Represented here is the Interneuron.

Neuron Function 
– Neurons are electrically excitable cells in the nervous system that function to process and transmit information. They are the basic working unit of the brain, a specialized cell designed to transmit information to other nerve cells, muscle, or gland cells. Most neurons have a cell body, an axon, and dendrites. The cell body contains the nucleus and cytoplasm. The axon extends from the cell body and often gives rise to many smaller branches before ending at nerve terminals. Dendrites extend from the neuron cell body and receive messages from other neurons. Synapses are the contact points where one neuron communicates with another. The dendrites are covered with synapses formed by the ends of axons from other neurons. In vertebrate animals, neurons are the core components of the brain, spinal cord, and peripheral nerves.
How action potential moves along the neuron fiber
The nerve impulse is a signal called an action potential. The action potential is an electrical charge that goes down an axon. The neuron can get input from other neurons from neurotransmitters and the action potential goes down the axon when it reaches the axon terminal it stimulates all of the other neurons.

 

The photo below is a photo of the Structure of a Synapse along with its parts labeled.

Synapse Function 

  • It’s the structure that permits a neuron to pass an electrical or chemical signal to another neuron or to the target effector cells a synapse, one neuron sends a message to a target neuron—another cell. At a chemical synapse, an action potential triggers the presynaptic neuron to release neurotransmitters. These molecules bind to receptors on the postsynaptic cell and make it more or less likely to fire an action potential.

How a signal is sent from the axon of sending neuron to the dendrite of receiving neuron

  • The axon of the sending neuron does not actually touch the dendrite of the receiving neuron. there is a tiny gap between them, the synaptic cleft, the neurotransmitters travel across the synapse between the axon and the dendrite of the next neuron.

How the receiving neuron “determines whether or not to send its own action potential.

  • The receiving neuron will determine whether or not to send its own action potential if it sees if it is excitatory or inhibitory. Excitatory is when the stimulates action potential on the receiving neuron and inhibitory is the repress’s action potential on the receiving neuron.

 

Journal #5 – Français 11

Je pense que le jeu était un succès. Je sens très contente et fière du l’effort que mon group a donné à ce projet. Nous avons pris du temps à l’extérieur d’école pour assurer que notre jeu á de la qualité. J’étais aussi contente que le produit du jeu était bon aussi. Notre travail était traduit avec les résultats. Il me sentait comme les autres groups autour de nous ont l’aimé aux causes d’énergie qui venait de leur table. Je pense aussi que nous comme un group étions très bonne. Molly et Brooke sont les très bons amis et pour moi c’était très amusant pour travailler avec un group des personnes qui sont très bons amis et aussi qui travaille très fortes et intelligentes. Je pense qu’un défi était le temps. Il y avait un fois ou Brooke était parti pour 2 semaines et puis Molly n’était pas le pour un weekend et puis je devais travailler sur le devoir des autres classes. Trouver du temps était difficile quelquefois. Mais à la fin, J’aime le jeu et mon groupe. Une très bonne expérience.

Final week Blog post – Luka Mladenovic

Hello,

It’s finals week, so it’s time to cram all that I can into my head so it hopefully sticks.

I found myself having an easy time remembering quadratic equations, trig, and absolute values. I decided to spend a bit more time on a topic that I was having a hard time trying to remember. The first unit which was Arithmetic/geometric series and sequences. I think my blog post from the very beginning came in handy because it was as if I was explaining the topic over again to myself in a way that I understood the most.

I have attached the link to the original YouTube video I made on Arithmetic/geometric series and sequences below.

Thank you for a great year, I feel like the strategy of working and studying in and out of class really helped me speed up my understanding of this course.

Luka.

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