DNA and Protein Synthesis

1. Explain the structure of DNA

DNA is made out of sugars (green pentagons), phosphate (blue circle), and nitrogen bases (colourful shapes). The nucleotides then coil around together to form a double helix shape. The complementary base pairing being Adenine to Thymine, then Cytosine to Guanine. DNA has 2 backbones, one going from 3′ to 5′, and the other 5′ to 3′. The bases, phosphate groups, and sugars are bonded with covalent bonds (straight lines), while hydrogen bonds form between the 2 bases (dotted lines).

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2. When does DNA replication occur?

DNA replication occurs right before cell division, or during the S-stage of interphase.

 

3. Name and describe the 3 steps involved in DNA replication. Why does this process occur differently on the “leading” and “lagging” strands?

The first step in DNA replication is unwinding and unzipping. This is done by DNA helicase. (In the picture, the ‘scissors’ is the helicase ‘unwinding’ the 2 backbones apart.)

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The second step is complimentary base pairings. This is done by DNA polymerase. Each A binds with a T, and G with a C. (In the photo it has just finished unwinding, and will now be paired by the polymerase).

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The third step is the joining of adjacent nucleotides. This is done by DNA ligase. It joins the newly created strands to the old one by using phosphodiester bonds. (The yellow arrow points in the direction that the joining goes.)

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The process for the leading strand is read by the DNA polymerase from the 3′ to the 5′ end. It is different from the lagging strands because the lagging stand is read from the 5′ to the 3′ end. But because the polymerase can only be read starting from the 3′ end, the complimentary strand is built in short segments moving backwards.

 

4. What did you do to model the complimentary base pairing and joining of adjacent nucleotides steps? In what ways was this activity well suited to showing this process? In what ways was it inaccurate?

To model the complimentary base parings, we used the shapes to match A-T and C-G. Then to join adjacent nucleotides, we used the yellow arrow to model the new backbone being made. I think our activity was well suited because it showed the general idea of DNA replication. It was also easier to understand the 3 steps. It was inaccurate for some of it because the real DNA strand would be way longer.

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5. How is mRNA different from DNA?

DNA has 2 backbones and is a double helix shape, while mRNA has only 1 backbone and is a strand. DNA’s sugar is deoxyribose while mRNA is ribose. DNA also has thymine while mRNA has uracil.

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6. Describe the process of transcription.

Transcription is the process when 1 of DNA’s backbone is copied to one strand of mRNA. Because one of the backbone form DNA has the correct instructions for building the protein, it is named the “sense” strand. The 3 phases of transcription include:

Unwinding & unzipping: Where the DNA unwinds.

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Complimentary base pairing: The nucleotides of mRNA are joined by the enzyme RNA polymerase, where it joins a complimentary base of DNA

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Separation: The inactive DNA strand will be separated.

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After transcription, it will be modified and then then leave the nucleus.

 

7. How did today’s activity do a good job of modelling the process of RNA transcription? In what ways was our model inaccurate?

We managed to model it unwind, and pair it with its complimentary bases, and also the separation. In the end, we also got it to show the old DNA strand separated (Picture). It was inaccurate because in the real thing, the size would be different and longer.

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8. Describe the process of translation: initiation, elongation, and termination.

Translation occurs in the ribosome, where the protein are synthesized. In initiation, the small ribosome sub unit binds to the mRNA sequence. In elongation, the ribosome holds the mRNA and then allows tRNA to attach to it.

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The mRNA has its 3 letter codon, while the tRNA will create a complimentary 3 letter anticodon.

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There are 2 tRNA’s, one that will bind to the ‘A’ site, and one will bind to the ‘P’ site.

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The amino acid will then let go of its tRNA and then binds with its neighbouring amino acid. Then the ribosome will shift so that the 2nd tRNA will be at the ‘P’ site, and a new tRNA will bind to the ‘A’.

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This process repeats until they reach the “STOP” codon. That is when termination will start, no new amino acid will be added, so the ribosome will then dissociate and release the polypeptides.

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9. How did today’s activity do a good job of modelling the process of translation? In what ways was our model inaccurate?

It managed to get the process of scripting out the codons and grouping them, and also the translation process with the ribosomes and tRNA. I think the process with the ribose moving along the strand, and the tRNA moving with it seemed pretty accurate to the description. The model was inaccurate because the shapes probably don’t look exactly the same as the real ones. And also the real ones would be way longer.

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Lit Circle Theme Park Project – The Back of The Turtle

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After we all brainstormed ideas for the theme park, we each did a section of it. I did the characters part, with the three characters being Gabriel, Dorian, and Sonny. The most challenging thing about that was trying to find pictures for the characters, since the book did not describe their appearance very much or even at all. So, I went through each character and tried to find even small details of how they look like. Sonny for example, they never said anything about how he looks like. But I remembered that if Crisp is Sonny’s uncle, they probably had to look somewhat alike. Crisp had reddish hair and blue eyes, so thats what I imagine Sonny to have too. The only thing the book said about Dorian was that he used to have long hair, so i’m assuming now he has short hair. Since he is the CEO, he probably dresses like one too. Gabriel was probably the more easy one out of all of them. At first, I tried to look for an First Nations guy for his photo, but then I remembered that in the book, its mentioned a couple times that he did not look Indian. He is said to have short brown hair, and pale skin. So I looked for a guy that matched that. I also tried to base their theme park personalities as close as I could do their book ones. To find out the personalities of the characters, I looked at their actions, what others say about them, and also what they think about themselves. I found lots of quotes to support it too. In doing this project, it really helped with knowing the characters better. Like how it was nice to find a picture that looked something like them. When your trying to put a person in a completely different environment, you have to get to know them really well to guess how they would act. The park shows, merchandise, and rides also matched the theme of the book quite well, and it would be really interesting to see it in real life.

Mind Map – “A Private Experience”

     “A Private Experience” by Chimamanda Ngozi Adichie is a short story about ethnic and religious conflict. The mind map looks into the story with seven different categories. The story is told in third person, but because you can see only Chika’s thoughts, it makes it third person limited omniscient. I chose the photo and quote in the mind map to show that. The physical setting for “a private experience” is first set in a marketplace in Nigeria. Later, Chika and the other woman hide in an empty shop when the riot happens. Emotional setting composes of fear, tension, and confusion. Chika does not know the woman, nor does Chika know why the riot happened. But because of the fear from the uproar, they hide together. The picture I chose is a market, showing the physical setting at the beginning of the story. The quote I chose shows the emotional setting of Chika when she looks outside. As you may already know, Chika is the protagonist of “a private experience.” We see multiple sides of her, in present and future self, making her a round and dynamic character. Dynamic because of the way the riot changes her views of the other woman. The antagonist of the story is the riot itself. Thus, making the conflict person vs society, or even society vs society. I chose the quote because it shows the roundness and dynamics of Chika. The theme for “a private experience” shows that even in times of religous conflicts, there will be some who still have amity. Chika and the other woman are from different religions, and even though the riot started because of religion differences, they both decide to set those differences aside and help each other. I chose this picture because it looks like two woman, with some sort of religion, just like in the story. I chose this quote because it shows that the other woman is Muslim because of her scarf, yet she uses it to help Chika’s leg. The plot of the short story starts from the two woman hiding together in an abandoned store, hiding from the riot. Through a series of flash forwarding and back, they soon get to know more about each other, and also learn why the riot had begun in the first place. When the riot dies down, they part ways with Chika keeping the woman’s scarf as a reminder of what they’ve been through. I chose this picture to show what the riot looks like. The quote I chose because it sums up the ending of the plot nicely. For the literary criticism lens, I chose psychological. We look at the psychological motivations of the character to find out more about the character. Even though theres only one riot going on, there are many different reactions that different characters have. So using this lens, it can better help analyze the characters. I chose this picture and quote because it takes a look at what Chika is doing, which helps us understand what she is feeling at that time. There were many symbols in this story. One of them being the scarf, which at the end symbolized the bond between the two woman. Chika kept the scarf because she wanted something to remember the woman, the riot, and the time they had together. The woman’s scarf also helped Chika when she was bleeding. I think the scarf also symbolizes friendship, and hope. Chika hopes that she finds her sister. The riot symbolizes violence. It started from the conflict of 2 religions, and then turned into something bigger. When there is violence, people will act in different ways. Some fight it, some leave it, and in this case, some hide from it. In the beginning, when Chika and the woman first meet, they say that they lost their stuff. Chika lost a bag, and the woman lost a necklace. I think those items symbolizes their differences. To lose those items, it represents putting those differences aside. That is why even though they are from different religions, they manage to respect each other and hide together.

     The main colours I put for this mind map were black, orange, and white. I did it black and orange to make it look like a riot, or like a fire from the riot. The white was so that the text could be seen clearly. The first ‘slide’ is an overview of all the different topics, including a picture. Then I made it go through all the different topics in different slides, putting that same picture. I did the description text black, and the quote text orange, so that it could be differentiated more easily. For the slides, I made it fill up the page with information, yet also make it simple enough so its not confusing.

 

 

 

 

 

 

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Visual Safety Guide

Tip #1: Do not run or shout in the lab. You may accidentally bump into someone and cause a disaster. And if you shout, the other people wouldn’t be able to listen to important information.

No Running/Walking Only Classroom Posters by LittlePeopleLove | TpTNo Running/Walking Only Classroom Posters | Etsy

 

 

 

 

 

Tip #2: Wear closed-toed shoes, tie up long hair, and don’t wear loose clothing. Wearing open-toed shoes can be dangerous if something like a knife falls, so thats why it’s safer to wear closed ones. If you have long hair or loose clothing, it may fall into the food or catch on fire, so thats why its safer to tie up your hair or wear fitted clothing.

Lab Safety Rules Guide Lab Safety Rules This

 

 

 

 

Tip #3: Wash your hands with soap frequently. Because of covid-19 it is important to wash your hands a lot, because there is food and people around so you want to make sure your hands are clean. With soap too so the soap can kill any bacteria you may have and slow the spread of the virus.

When and How to Wash Your Hands | Handwashing | CDC

 

 

 

 

Tip #4: Be careful when using knifes. Don’t put them in the sink with soapy water, because it may accidentally cut someone. Also when holding knifes, make sure the pointed side is down so it doesn’t cut someone.

12 Kitchen Knife Safety Tips

 

 

 

 

Tip #5: If you break or spill something, make sure to clean it up properly. If you spill water, wipe it up with paper towels, or else someone may slip. If you break glass, make sure to dispose of it right or else it might cut someone.

Helpful Tips for Cleaning Up Broken Glass | Image Glassworks

Physics 3D Print Project

Hi,

So in Physics 11, we had to choose a question from the book, then use a 3D printer to create a model for that question. The question I chose was about a person swinging on a rope (below). I actually changed my question and design a lot, but eventually I chose this one.

Question: A student is running at a speed of 3.5m/s and grabs a long rope that is hanging vertically from a tree. How high can the student swing?

Answer: 1/2( 3.5^2)=-(9.81)(-h)

h = 0.6m

As you can see, there is a round object connected to a golden chain which is connected to the ‘tree’ which is a bar. The student (green figure) runs up to it, then swings and lands on the other side. The speed is 3.5m/s and so I just put it in the equation [1/2(v)=-(g)(-h)] and got my answer.

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Logo Design

Ideas for your Logo and how they connect to you 

My original ideas ended up being very different to my final. But from my original ideas, I built and changed things from that which eventually made it into what it is now. At first I starting sketching simple designs/shapes, but then I thought about doing an animal because I like animals. It took a while to decide with animal I should do but I ended up choosing the jackal. Here is the photo I used for tracing.

Research 

When deciding which animal I was going to do, it took a very long time. I first thought of doing my favourite animal, but then it was too hard. Then I kept thinking about it, and remembered there was this book I used to read, and there was many animals including the jackal in it. The one in the book was not the main characters but it was cool and I remember it. So then I started researching more about the meaning.

“Jackals represent tricksters, inventors, youth, fun, intelligence, and tactical people. Many legends which are found all over the world implies that a jackal is a lone animal that walks in forests and deserts.”

The Results – 1 Black and White and 2 colour 

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Reflection – What makes it successful 

I think it is good. I like it.

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Basket Golf

Riverside Game Design

Name of the Game
Basket Golf
Objective – What is the Purpose | Goal of the Game
  Objective is to get the most points with your 10 balls within the 30 second time limit. 
Description – Describe Your Game and What it Should Look Like
This game should be played with at least a minimum of 3 buckets to a maximum of about twelve buckets. Place the buckets  at least six feet or more in front of  you on the floor or on a raised surface and at least a  foot or more apart  in a horizontal or vertical manner. The buckets can be colour coded or they can be identified with numbers.The  1st bucket(s) should be 5 points, the second should be 10 points, and the third bucket(s) should be 15 points and so on. For each additional bucket(s) you can increase the point by 5 points. The buckets can be the same size or different sizes, it depends on the player and how challenging you want the game to be.

 This game can be played by anyone of any age but in order to help younger children between the ages of 3-8, with their coordination, you may want to use larger buckets and put them in closer proximity to the children. In order to increase the complexity of the game you can add buckets of the same size or different sizes and lay them out randomly or in multiple rows.

 For example, you can have four small buckets at five points, four medium buckets at ten points and four large buckets at 15 points. You can set the buckets out randomly amongst each other or you can set each size of bucket in a horizontal or vertical row.

You can use soft balls, bean bags, foam balls, kids toy balls, or crumpled paper. 

If players do not have balls or buckets to play the game, the game can me modified by using  plastic cups and crumpled paper instead of buckets and balls

Rules of the Game – Describe the Rules

Any number of people can play this game

This game can be played with each player participating  one at a time or by having two or more players participate at the same time.

If a player gets a point  after the time limit it will not count

During the time of covid remember to adhere to the 6 feet distancing rule

For teens and adults playing the game remember to try and remain at least 6 feet  away from the closest bucket 

Each person should have 10 balls and a 30 second time limit (the number of balls can be changed)

Strategies | Tactics  Progressions – Can it Evolve
A strategy is to try and perfect your aim, you don’t want to throw the ball too far, or too close. You want the aim to be just right so it gets into the bucket.

You can throw your first one, and based on how far it goes, use that to measure how hard or soft your next ones will be.

To develop your eye hand coordination and to challenge yourself, you can try standing farther away from the buckets.

If a player(s) does not have the required items they can try using  something as simple as  the pockets of a shoe holder or plastic cups

Another idea is to add more buckets so that it challenges you  to move and think faster to get more points within  the time limit. To increase the level of competition for multiple players, add additional buckets  and have  everyone play in a circular manner while trying to score the most points within the time limit.

Equipment – List What is Needed Safety Considerations – Safety First
 10  balls ( i.e.  tennis balls or golf balls), bean bags or styrofoam balls,  

3 or more buckets or plastic cups

One or more players

 A marker (to indicate the number of points each bucket is worth) Or colour coded buckets or plastic cups

 Some method of timing yourself

Make sure you are socially distancing from the person in front of you.

Wear gloves if you choose to share the balls with each other. Or use/ bring your own balls.

Play the game outside or in an area without any breakable items.

 To reduce safety concerns is to  use soft balls,,bean bags or styrofoam balls

 

 

REFLECTION

I think this project went well. The planning and video ended up good. My role in the project was editing the video and helping with the planning part fill out thing. Overall everything went pretty smoothly in my opinion and it was good. Since I had edited a lot of videos before making this one wasn’t that hard and I knew what to do. We made a google docs so we could all edit the document above, so that went well and we got that finished. To improve for next time, we can fix some things from the feedback we got. Like the time limit may be too short to throw all the balls in, or stuff like if it bounces in but then bounces out would it still count. For our game, they also said that they liked that it was simple enough to understand yet challenging enough to be fun. And also that the video was good.

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