Lactase Lab

Experimenting with temperature and lactase

Cam, Joy, Anna, Jaxson

April 18th, 2019

Purpose:

The purpose of this experiment is to see how temperature affects the rate of an enzyme driven reaction. By heating each test tube to a different temperature, we are hoping to get different results on a Diastix

Hypothesis:

If the temperature of the water is increased, then the Diastix will read that there is more lactase in the milk because warmer temperatures are ideal for the enzyme lactase to be in to work best.

Materials:

  • 1 Beaker
  • 6 Test Tubes
  • 6 Test Tube Stoppers
  • Milk
  • Hot Plate
  • Lactase
  • Diastix

Methods:

  1. Fill all test tubes with exactly 10 mL of milk and place in test tube holder (Make sure they are labeled)
  2. Fill one beaker with 100 mL of water
  3. In each test tube add 3 drops of lactase
  4. In the first test tube dip one of the Diastix to get a base result
  5. For the second test tube set the temperature of the hot plate to 7 and heat up the water to roughly 25 degrees and let the test tube sit in the water for 3 minutes.
  6. After 3 minutes has passed dip the Diastix into your test tube and collect your data.
  7. Repeat this process for the rest of the test tubes (3 – 6) and increment the temperature 5 degrees

Results:

 

Data Analysis:

As you can see in the graph the comparison between Temperature and Concentration of Lactase it starts relatively low in test tubes 1 & 2 however as the temperature was increased the Level of Lactase increased and proceeded to stabilize at roughly the same level (3 – 6).

Conclusion:

Overall, our hypothesis ended up being correct and with that the lab was very informative in how enzymes can work better in warmer temperatures. The evidence is clear that as you raise the temperature the concentration of glucose raises.

Error/Improvements:

To improve on this lab, some things we should have changed were things such as the number of drops used in each test tube and the amount of milk that was put in the test tubes. We agreed that the number of drops should have been decreased by 1 and the amount of milk increased to 25 mL of milk instead of the original 10 mL

Desmos Art Functions Card 2018

Desmos Link- https://www.desmos.com/calculator/ytpf3kyar9

One of the challenges I experienced was the shading part. It took me awhile to figure out how to shade. Some of the aha moments included me trying figure out the eyes (My guy looks scared). I got help from a few friends. One of my strategies was to just punch in lots of different numbers to see what looked best (may not be the best strategy). This assignment made me how much I hate functions and relations,  but it also helped me understand the relationship between all of them.

Blog #17

Throughout this unit we not only reviewed trig but also learned new lessons. SOH CAH TOA can be rewritten as sin y/r, cos x/r and tan y/x.

we learned reference angles, which I find are easiest to solve when using a visual, such as a graph. The reference angle is determined by the closet x-axis, this will help decide whether or not you add or subtract from the degree of each quadrant.

 

we also learned the 2 special triangles. one we got by cutting a square in half and the other by cutting an equilateral triangle in half, these are used when we are asked for an exact ratio.

lastly we learned 2 new formulas, sin law and cosine law, these are used to solve non-right angle triangles, one or the other can only be used by fitting certain requirements

 

I tried to post the picture but my edublog isn’t working, so I will email your the picture.

Blog #15

This week we were taught how to multiply ugly fractions, we were taught how to do this during grade 9 and 10, but this week we brought in a lot of new concepts. We were taught to eliminate some terms that were the same on top as they were on the bottom and we also learnt about many other tips.

 

Jaxson 🙂

Blog #13- Recipricals

This week we were taught about reciprocals.

In this blog I will be teaching you how to draw reciprocals for a linear graph.

First step: Plot your points

Second step: figure out the x intercept

Third step: find the -1 and 1 on the y axis

Fourth step: graph the reciprocal

Fifth step: find the points

 

Blog #12- graphing absolute values.

This week in Pre-Calc 11, we learned how to graph absolute values. These can be applied to both linear and quadratic functions. This task is very easy to complete.

When graphing absolute values the quatratic or linear line will never go below the x intercept.

When the parabola has a absolute value it forms a W shape.

This all happens because absolute value can never be a negative.

 

 

 

 

Pre Calc 11 – Blog #11

This week we learnt about systems of equations. A system of equations is a collection of two or more equations with a same set of unknowns. In solving a system of equations, we try to find values for each of the unknowns that will satisfy every equation in the system. The equations in the system can be linear or non-linear.

First step: Factor out the equation

Second step: Find the zeros

Last step: Test the numbers to see if your equation is true


Blog 5 – Trinomials

This week in pre-calc we learned how to factor using trinomials.

There are a couple steps to figuring this out, but once you have figured it out it is very simple.

  1. identify a,b, and c in the trinomial ax2+bx+c.
  2. write down all factor pairs of c.
  3. identify which factor pair from the previous step sums up to b.
  4. Substitute factor pairs into two binomials.

example:

  1. Continue reading