Bio 12 Lab – Lactase Drops vs Glucose Production
April 18th,2018: Lactase Vs. Lactose [ ] lab
Experiment: Same glucose solution mixed with a different number of drops of lactase:
Purpose: To see the effect an increased or decreased amount of concentration of lactase enzyme mixed with a dairy substance will effect the rate of production of glucose within a reaction
Hypothesis: If more lactase drops are added into the milk, then the greater amount of glucose is produced, because more of the enzymes that break down lactose are working in larger amounts to produce different amounts of glucose.
Materials:
- 5 Test Tubes
- Test Tube Rack
- Milk (2%)(60ml, 12ml per test tube)
- Lactase Drops
- 10 Glucose Strips
- Graduated cylinder (50ml)
Procedure:
- Prepare 5 test tubes with the required amount of milk (30 ml per test tube).
- Prepare the lactase dropper and drop the required amount of milk into the test tubes except for one, this is your control tube
- Test each solution with a glucose strip before adding any drops, and record information in data chart below.
- According to the chart below, drop the required amount of lactase drops into the milk.
- Make sure the solution is mixed well with the milk.
- Wait 10 minutes before testing each test tube with the glucose strips.
- Once it has been 10 minutes, place a glucose strip into each test tube wait 30 seconds, compare colour to chart below and record the data below.
- Graph your data, [ ] of Glucose vs. # of Lactase drops, with # of Lactase drops on x-axis, and [ ] of Glucose on y-axis
Data/Observations:
|
Test Tube # |
Lactase Drops |
Initial Glucose (Strip colour + mmol/L) |
Final Glucose (Strip colour + mmol/L) |
|
1 |
0 |
Negative |
Blue , 0 mmol/L |
|
2 |
2 |
Negative |
Light brown, 56mmol/L |
|
3 |
4 |
Negative |
Medium brown 56mmol/L |
|
4 |
6 |
Negative |
Medium brown 56mmol/L |
|
5 |
8 |
Negative |
Dark brown 111mmol/L |
Analysis/Conclusion:
In this lab it could be inferred from the data table that as we added more lactase drops the rate and breakdown of lactose into glucose was increased and therefore it can be said that they are directly related (add more of one produce more of the other through breakdown). Due to the low volume of milk that we used, our hypothesis cant 100% really support our findings because almost all the results were very similar, so no facts were able to truly be compared based on the similarities within the colour indicators (some of the tubes with more drops of lactase had the same colour indicator change). However in generalization with more lactase drops a higher amount of glucose found then starting levels. Therefore it can be concluded that the more lactase added to the milk changes (higher) the production of glucose within the milk substance.
How do you think you could improve this experiment for the most accurate outcome? (Errors/Improvement) Ways that would be most affective in improving this experiment would be having a greater volume of milk. This would have made the results show a larger difference of change greatly due to the concentration of the lactase drops to milk being lower than what they were in this particular experiment, changing the results so they had visible differences within the colour indicator scale, and therefore giving a more accurate reading.
By : Alex, Molly and Sophie